Protocol for the preparation and application of a Trichoderma harzianum based bioinput for family farmers
ADVANCES IN HORTICULTURE - REVIEW | Sánchez, A. - Stocco, M. - Mónaco, C.
Tags: family producers, biocontrol, diseases, sustainable management, vegetables
One of the biggest challenges for the production of bioinputs is that the product is adequately developed. This implies that it contains a sufficient amount of inoculum and that its quality is maintained during storage time. In this sense, the objectives of this work were i) to establish a protocol for the preparation and application of a bioinput based on Trichoderma harzianum and ii) to develop a protocol for family producers on the correct use of the bioinput produced. For the multiplication of T. harzianum, rice grains were used as a solid substrate. Polyethylene bags were prepared with 30 g of rice plus 15 mL of sterile distilled water. The bags were sterilized and seeded with a culture of T. harzianum. The bags were incubated at room temperature for 10 days. Once the rice was colonized, it was dried in an oven at 50° C for 24 h. The quality was evaluated one month, three and six months after the preparation of the solid product. To do this, the concentration of conidia was calculated by counting in the Neubauer chamber and the viability of the product was also calculated by counting colony-forming units of Trichoderma in Trichoderma selective medium culture medium. The results obtained indicate that the concentration of conidia in the solid substrate remains high up to six months of storage. A protocol of use was developed for producers.