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The aim of this work was to evaluate the effects of aqueous extracts of rhizomes of Sorghum halepense on mycorrhizal colonization, succinate dehydrogenase activity, total dry weight, relative conductivity cell membrane and net photosynthesis in Mentha x piperita plants. Rooted cuttings non inoculated or inoculated with Glomus intraradices or Glomus mosseae grown during 60 days into an inert tyndallized substrate, irrigated with Hoagland solution and four concentrations of aqueous extracts of Johnson grass rhizomes: 0 %, 5 %, 10 % and 15 %. The experiment was carried out in a greenhouse, in 3 x 4 factorial arrangement, in completely randomized design. The higher mycorrhizal colonization occur in treatments without Johnson grass, 87 % and 92 % by G. mosseae y G. intraradices respectively, and decreasing significantly with the higher doses of extract. The fungus structures viability decreasing significantly with the higher doses of extract, and the mycorrhizal dependency increased by 35 % with the highest dose of extract. The higher concentrations of extract reduced the total biomass and photosynthesis, and increase the relative conductivity the membranes of leaves and roots. The inoculated plants showed a better performance than non-inoculated. Aqueous extracts of Johnson grass decreased plant growth while inoculation with mycorrhizal fungi reduces adverse effects.